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About Me
Retired Hospital Scientist. ( worked in hospital Pathology Departments ) Live in Sydney, Australia. Hobbies are photography, bushwalking, manual scrapbooking, making items with photos online with Snapfish, x-stitch, reading, watching TV documentaries. I have a boyfriend, with whom I go out as much as is possible - we share lots of things in common - watching movies, walking. We used to go caving, canyoning, but these things are pretty well out of my reach these days. I've just taken up digital scrapbooking, & have to admit I'm addicted - but that's OK. I manage to mostly fit other stuff into the day, that needs doing. I have a feathered baby called Monty. He's a very talkative, inquisitive cockatiel, & I would totally miss him if anything were to happen to him. I have to scrap while he's in the cage, otherwise he's on the keyboard, removing the keys! Jesus is my Lord & Saviour, & my Home is Heaven - I'm very much looking forward to being there with Him.
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Digital Scrapbook LayoutDescription
This flow chart tells how bacteria are cultured, identified, & their sensitivity to antibiotics determined. (1) A swab is rubbed on the suspected pathogenic site, eg. a leg ulcer. The material on the swab is then lightly rubbed on the surface of bacterial growth media. This media could be in petri dishes or in test tubes.( This is called Inoculation of media. ) The inoculated media is then incubated over night. (2) The next day, the inoculated media is inspected for the growth of bacterial colonies. If there is growth, a suspected pathogenic colony/ies is/are picked off the media, & placed in liquid growth media for the morning. In the afternoon, special media that contains reagents & an indicator, are inoculated from the morning's culture. These special media cultures are incubated over night. (3) On day 3 the bacterium/ia can be identified, by how they metabolise, & produce certain chemicals, which then turn the indicator a certain colour. Other tests based on the structure of the bacteria cell can also be carried out to identify an organism. (4) A special medium in a petri dish is then inoculated with the liquid culture of the organism, & special blotting-paper-like rings with discs that are impregnated with different antibiotics, is placed on the surface of the medium. This petri dish is then incubated, (4) then inspected for antibiotic sensitivity or resistance. If there is a wide region around the antibiotic disc that has no growth of the organism, that bacteria is regarded as being sensitive to the antibiotic. If there is no clear region around the disc, or just a small region of clear media around it, then the bacteria is considered resistant to the antibiotic.
This is how things were done when I first trained in Bacteriology. Now, most of this manual work is done by machines & analysers. For this LO, I used Mad Scientist by Melo Vrijhof from PS, Love the 80's By Digi Treats from Etsy, & Lily of the Valley by Kastagnette from Digital_Crea.fr I also used clipart from Imgbin.com
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